Coding

Part:BBa_K2665016:Design

Designed by: You Tong   Group: iGEM18_Kyoto   (2018-10-08)


TDH3-AtHKT1-6xHis-CYC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2238
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1376


Design Notes

We added promoter and terminator in order to express the protein, and added His-tag for Western Blotting.


Source

from Arabidpsis thaliana.

References