Coding
Part:BBa_K2665016:Design
Designed by: You Tong Group: iGEM18_Kyoto (2018-10-08)
TDH3-AtHKT1-6xHis-CYC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2238
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1376
Design Notes
We added promoter and terminator in order to express the protein, and added His-tag for Western Blotting.
Source
from Arabidpsis thaliana.